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文献和实验Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines
Mesenchymal cells have been cultured successfully for many years owing to the ease of their culturability. With the advent of more complex growth media, and some changes to methods, it has become possible to culture epithelial cell types
from its endogenous mesenchyme, undergoes branching morphogenesis when cultured ex vivo in a basement membrane extract in serum-free medium with growth factor stimulation. The resulting three-dimensional epithelial morphogenesis in the defined culture system makes
Culture and Differentiation of Mouse Tracheal Epithelial Cells
Airway epithelial cell biology has been greatly advanced by studies of genetically defined and modified mice; however it is often difficult to isolate, manipulate, and assay epithelial cell-specific responses in vivo. In vitro proliferation
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