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文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
(agarose coupled) gtm-20 (magnetic particles) (®ChromoTek, Martinsried, Germany, www.chromotek.com) Back to top Reviewer Comments Reviewed by: Raffaella Villa, Laboratory of Peter B. Becker, Adolf-Butenandt
-acetate, pH 7.5 0.3 M NaCl 0.5% IGEPAL CA-630 0.1% sodium dodecyl sulfate (SDS)5 0.02% NaN3 Store at 25 C indefinitely Protein A-Sepharose CL-4-B Suspension6 Protein A-Sepharose beads (Pharmacia or Sigma) are swelled in PBS or IMP buffer on a tube
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
dodecyl sulfate (SDS)5 0.02% NaN3 Store at 25 C indefinitely Protein A-Sepharose CL-4-B Suspension 6 Protein A-Sepharose beads (Pharmacia or Sigma) are swelled in PBS or IMP buffer on a tube turner at room temperature. Beads are then washed
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