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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Organ Culture dishes (60 x 15 mm, Falcon, 3037); M2 and KSOM media. Method: The oviducts with the upper part of the uterus attached are removed from 2.5 days post-coitum (dpc) superovulated CD-1 females and placed into a drop of M2.
The recent discovery of genomic reprogramming of human somatic cells to an embryonic stem (ES) cell-like pluripotent state provides a unique opportunity for stem cell research. The reprogrammed cells, named as induced pluripotent stem (iPS
Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood
of the reprogrammed cells, the culture conditions for serial passages of iPS cells, and the protocol for cryopreservation of established iPS cells. As the efficiency of gene transfer to suspension cells is relatively low compared with adherent cells
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