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Expression of EGF and HIV Envelope Glycoprotein

This chapter reviews the expression of two proteins that are naturally secreted: murine epidermal growth factor (mEGF) and the 120-kDa envelope protein from human immunodeficiency virus (HIV-1 ENV). Although the initial aims of these projects

Isolation and Culture of Human Astrocytes

Although rodent models have been essential to unveil the emerging functions of astrocytes, the existence of interspecies differences calls for caution in extrapolating data from rodent to human astrocytes. We have developed

Mutational Analysis of the Human Protein C Gene

The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes