pharmacia特约代理商|17042001 THIOPR

Sepharose CL

相关专题   (一)　原理 　　葡萄球菌A蛋白(SPA)具有与多种哺乳动物 IgG分子Fc段结合的能力，并与不同IgG亚类的结合力有所差别。利用改变PH及离子强度可洗脱结合于SPA-Sepharose CL-4B柱上的IgG或不同的IgG亚类，可直接纯化血清或小鼠腹水中的IgG抗体。 　　(二)　操作步骤 　　用0.1M PH8.0 磷酸缓冲液 浸泡SPA-Sepharose CL-4B凝胶，15min。按1g干胶200ml

Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose

in outcomes. The procedure provided below has proven to be highly reproducible. It is particularly effective for the purification of m-calpain, although it is also useful for μ-calpain. Others have described the use of Blue Sepharose-CL-6B for the purification

PREPARATION OF PROTEIN A SEPHAROSE CL 4B BEADS

so that the total volume of beads plus buffer is 2 ml).Blocking and Use1.Remove desired volume of 50% slurry (30 µl of slurry per I.P.or Chip).Put in 15 ml conical tube.2.Spin in table top3.Resuspend beads in cold blocking buffer:T. E.25 ml of T.E0.1% Azide25 µl