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上海玉博生物科技有限公司
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文献和实验Revised protocol for establishing a mammary cell line from tumors
Revised protocol for establishing a mammary cell line from tumors Drench mouse in 70% ethanol, pin and dissect open Sterile dissection of tumor- leave some behind to use in histology Place tumor in small culture dish Weigh
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
3) Transfer cells to an RNase-free centrifuge tube and pellet by centrifugation at 300 x g for 5 min. (In our lab, use 1800rpm in the eppendorf centrifuge 5415D). Cell pellets can be stored at –70°C for later use or used directly in the procedure
α response, we used two human-tumor-derived cell lines: the melanoma line ME-15 (10 ) and the hepatoma line HuH7 (11 ). We have chosen these cell lines as models, because we have a good understanding of the IFNα responses at the mRNA and the protein levels
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