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文献和实验Interaction Trap/Two‐Hybrid System to Identify Loss‐of‐Interaction Mutant Proteins
interactions play a critical role in biology. Disrupting a specific protein?protein interaction and studying the resulting phenotype can help elucidate the function of the interaction. A method to rapidly make and identify mutant proteins that no longer bind
Size‐Exclusion Chromatography with On‐Line Light Scattering
the use of size?exclusion chromatography with on?line light scattering, UV absorbance, and refractive index detectors (SEC?LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait
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