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文献和实验. Genes expressed in human tumor endothelium. Science 289, 1197–1202 (2000) | Article | PubMed | ISI | ChemPort | 30. Huang, X. et al. Tumor infarction in mice by antibody-directed targeting of tissue factor to tumor vasculature. Science 275, 547–
; stippled line, control staining without primary antibody. Screening for upregulation of target genes in human cell lines. We delivered millions of TFZF s into the human squamous carcinoma cell line A431 using a retroviral vector, pMX-IRES-GFP15 (Fig
5. Wash 4 times for five minutes per wash in TBST at room temperature. 6. Add 500 µl of diluted alkaline-phosphatase conjugated secondary antibody and incubate for 30 minutes at room temperature with shaking. (Sigma anti-human IgG conjugate
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