VWR特约代理商|53503-612 VWR® and VW

CHIP PROTOCOL FOR YEAST

sec each. Spin in microfuge at maximum speed, 10 minutes, 4°C-10°C (TOMY @ 15K 18,000 x gmax). CHROMATIN SOLUTION Transfer sup (might seem cloudy from the SDS) to 15 mL Falcon snap-cap tube on ice (expect ~1.1 mL). (Tubes: VWR cat

CHIP PROTOCOL FOR YEAST

) to 15 mL Falcon snap-cap tube on ice (expect ~1.1 mL). (Tubes: VWR cat. # 60818-689) Add 10 mL IP Dilution Buffer with 1 mM PMSF, 0.8 µg/mL pepstatin A, and 0.6 µg/mL leupeptin (10 mL 9 volumes SDS lysate; final [SDS] 0.1%). Let sit