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文献和实验sequences will be specific for the primary transcript (see Robert Singer's web site for more details on use of oligos as probes: http://singerlab.aecom.yu.edu/). For the detection of Xist RNA coating of the X chromosome in cis, or primary transcripts of X
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
of water, or (ii) 8g agar, 18g dried yeast, 10g soybean meal, 7g molasses, 80g malt extract, 80g cornmeal and 6.3ml propionic acid in 1lt of water). Facultative: to improve growth conditions, you might add a large drop of live baker's yeast
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
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