biomol特约代理商|223374.50-50 µl A

Overview of Receptor Allosterism

.    Bruns, R.F. and Fergus, J.H. 1990. Allosteric enhancement of adenosine A1 receptor binding and function by 2‐amino‐3‐benzoylthiophenes. Mol. Pharmacol. 38:939‐949.

Characterization of Histaminergic Receptors

2 [3 H]R ‐α‐methyl histamine Agonist 0.2‐0.8 20‐50 Moderate Moderate Amersham [3 H]N ‐α‐methyl histamine Agonist 0.2‐0.8 45‐90 Low High Perkin‐Elmer SNR in tissue‐based assays > RαMH [125

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

(Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed followed by a pulse