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上海玉博生物科技有限公司
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文献和实验Construction and Characterization of Adenovirus Vectors
), 1.25 g/mL and 1.35 g/mL, in dialysis buffer Prepare each gradient solution separately. Sterilize by filtration through a 0.2-µm filter. Chloroform:isoamyl alcohol (24:1) Citric saline (2X) For 1X citric saline, dilute the 2X
Culturing Chick Embryonic Myoblasts/Myotubes and Fibroblasts
plates. Day 3 Materials 1. Saline G, in 34o C water bath. 2. Soybean trypsin inhibitor solid. 3. Medium, as for day 2, on ice. 4. 3x crystallized trypsin in saline G, on ice. 5. Millex-GV filter and syringe
to Minicolumn Reload syringe with DNA sample which has been resuspended in low salt buffer (5, 10 or 20 ml). Attach the Elutip pre-filter to the column. Attach syringe to pre-filter and column and slowly pass all of the sample through the filter
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