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上海玉博生物科技有限公司
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Sample buffer, one 60‐ml bottle Wash buffer, phosphate‐saline concentrate, 5×, one 100 ml bottle: transfer contents to a graduated cylinder, bring volume up to 500 ml
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
Immune Response Biomarker Profiling
Forceps and deionized water 实验步骤 Preparing buffers 1) Prepare 100 mL of the blocking buffer by mixing 5 mL of 1 M HEPES, pH 7.5, 4 mL of 5 M NaCl, 800 l of 10% Triton® X-100, 50 mL of 50% Glycerol, 610 mg
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