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文献和实验Sephadex G-50 spun column purificati
volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing. 1) Plug a 1ml syringe barrel with siliconized glass wool and fill to the top with a sterile suspension of Sephadex G50 equilibrated with H2 O. 2) Once filled place
Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing. 1) Plug a 1
Method for Single-Cell Electroporation
) and found that concentrations ranging between 0.2 - 2.0µg/µl yield comparable numbers of fluorescent cells, with similar intensity of GFP fluorescence. DNA can be diluted in dH2O, buffered saline, or 2mM CaCl2. The DNA solution was colored with 0.01% fast green as a visual aid
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