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文献和实验An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis
Epitope mapping of proteins defines the structural elements of a molecule needed for recognition by individual antibodies. X-ray crystallography of anti- body-antigen complexes ( 1 ) is perhaps the most precise means of defining an epitope
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
to which are bound the antibody and the antigen, aspirate supe, and resuspend by vortexing in 500 microliters lysis buffer. 9) Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning and resuspending. 10
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
down bugs to which are bound the antibody and the antigen,aspirate supe,and resuspend by vortexing in 500 microliters lysis buffer. 9)Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning
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