invivogen特约代理商|tlrl-eklps Stan

One-step Transformation of E. coli

with 10% PEG (MW3350-8000),5% DMSO,and 20-50 mM Mg2+ (MgSO4 or MgCl2)at a final pH of 6.5]. 3.For long-term storage,cells are frozen immediately in a dry ice/ethanol bath and stored at -70℃. For transformation,a 0.1 ml aliquot of cells is pipetted

ChIP-Chip E. coli

of the genomic location on the X axis versus the ratio of the two dye signals (test/control). For OGT array 010010, the genomic location of the probes on the file is based on the EMBL E.coli K12 MG1655 genomic sequence (ID U00096). For OGT array

Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels

  Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels DNA Preparation E. coli chromosomal DNA is prepared following the method of Heath et al. ( J. Bacteriol., 174, 1992). Cells are embed allowfullscreen='true'ded in agarose