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Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

A  c-Kit (2B8) FITC-IgG2b, 01904D  FITC-IgG2b Isotype control, 11184C  Fc RII block (clone 2.4G2), 01241D   Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated

凋亡形态学图片

，被pi染色后能被共聚焦显微镜观察。本片显示的绿色荧光（外源性凝集素）勾勒出凋亡细胞表面的小泡，红色荧光（pi）被包被在其中。 Apoptosis induced in Jurkat cells with 10 µM camptothecin. The cells were then treated with the reagents in the Vybrant Apoptosis Assay Kit #4 (V13243). Apoptotic cell nuclei

白介素33信号通路图

of NFκB, p38 and JNK, as well as Myd88-dependent activation of ERK. JAK2 has also been shown to be involved in NFκB activation. Crosstalk of IL-33 with c-kit signaling has been proposed to enhance the activation of ERK, JNK, PKB and STAT3 in mast cells