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上海玉博生物科技有限公司
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文献和实验BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
实验试剂 Regents supplied by user 1. Sterile Deionized Water (or TE Buffer) 2. 70% Ethanol 3. 96%-100% Isopropanol 实验设备 Equipments supplied by user 1. Microcentrifuge capable
BACs, PACs, P1s and Plasmid Isolation Protocol (For low copy number)
3. 10-15 ml Culture Tubes 实验步骤 1. Isolate a single colony from a freshly streaked selective plate, and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
实验步骤 1. Isolate a single colony from a freshly streaked selective plate, and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20-24 hr at 37°C
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