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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
is less than 1 mm in diameter. A. Deparaffinization: Xylene 2X 3 mins each. 100% ethanol 2 mins. 95% ethanol 2 mins. 70% ethanol 2 mins. 50% ethanol 2 mins. dH2O 2 mins. B. Staining: 0.1-1% Methyl green
Alcian green stain for cartilage
% ethanol, several hours to overnight each. 6. Clear by washing 1-3X with methyl salicylate. Alcian green stain: 0.1 g Alcian green 70 ml 100% ethanol 1 ml conc. HCl 29 ml dH2O (100 ml)
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
. A. Deparaffinization: Xylene 2X 3 mins each. 100% ethanol 2 mins. 95% ethanol 2 mins. 70% ethanol 2 mins. 50% ethanol 2 mins. dH2O 2 mins. B. Staining: 0.1-1% Methyl green (dip 3-6 times or leave ~15-60 seconds). The section should not be too dark
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