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上海玉博生物科技有限公司
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文献和实验Isolation of cell nuclei for the application in the cell-free system
from the surrounding cytoskeleton during cell lysis. For the preparation of Jurkat nuclei, the step of cytochalasin treatment can be omitted. Input of about 1E+07 cells. Lysis in hypotonic medium (about 23 mM compared with 137 mM in isotonic medium) using a Dounce
in the natural microenvironment. Utilization of LCM for the molecular analysis of cells from skeletal tissues will enable one to study differential patterns of gene expression in the native intact skeletal tissue with reliable interpretation
17,178 −0.07 19,226 17,409 −0.10 14,425 14,079 −0.02 hsa-miR-19b 13,365 9,406 −0.42 25,463 22,438 −0.13 21,532 20,625 −0.04 18,039 17,440 −0.03 hsa-miR-30e-5p 9,497 6,838 −0.39 13,045 11,321 −
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