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文献和实验Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
LIVE/DEAD® Violet Viability/Vitality Kit
of labelings: At the recommended reagent concentrations and volumes, this kit contains sufficient material to perform approximately 200 assays using flow cytometry. Approximate fluorescence excitation/emission maxima: calcein violet 400/452 nm; aqua
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Modified Bradford assay kit (Bio‐Rad #500‐0002; also see unit 10.1 ) SDS sample buffer (see recipe ) Antibody raised
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