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LINEAGE ANALYSIS USING RETROVIRAL VECTORS

through a 0.45 mm filter and concentrated by centrifugation in an SW27 rotor at 4°C, 20K, for 2 hours. The concentrated stock was titered on QT6 and tested for helper virus, which proved negative. The titer of CHAPOL was determined to be 1.1 x 107 CFU/ml

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) Chloroform (Fisher) N ‐D‐(+)‐Biotinyl‐4,7,10,13,16‐pentaoxa‐1,19‐diaminononadecane ( 10 , Berry and Associates)