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上海玉博生物科技有限公司
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文献和实验His-tag protein purification using Ni-NTA magnetic beads
His-tag protein purification using Ni-NTA magnetic beads Materials and reagents Extraction buffer 20 mM Tris-HCl, pH 8.0 1 mM PMSF 1 mg/ml lysozyme 0.05
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
centrifugation (3000-5000 x g (6600 rpm) for 1 min)), and remove supernatant (use gel loading tips to completely remove supernatant). (23) Add 1 ml of ice-cold Low Salt Immune Complex Wash Buffer (EZ ChIP kit). Resuspend the packed agarose beads by gentle
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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