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上海玉博生物科技有限公司
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文献和实验His-tag protein purification using Ni-NTA magnetic beads
His-tag protein purification using Ni-NTA magnetic beads Materials and reagents Extraction buffer 20 mM Tris-HCl, pH 8.0 1 mM PMSF 1 mg/ml lysozyme 0.05
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
Mouse B cell isolation with magnetic beads
separation Positive :3 ml MACS for equilibrium, then cell solution, then wash 3x3ml followed by 2x4ml . Put the column on a 15 ml tube and flush the column with 5ml; Negative : 3 ml MACS for equilibrium, then collect the elution
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