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Lysosomal Acid Lipase: Assay and Purification

of intracellular lipid metabolism by three mechanisms: (1) free cholesterol reduces the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA)-reductase, the key enzyme of cholesterol synthesis (4 ) (2) cholesterol activates its own esterification by acyl-CoA

Techniques for Measuring the Activity of Carboxylic Acid:CoA Ligase and Acyl‐CoA:Amino Acid N‐Acyltransferase: The Amino Acid Conjugation Pathway

100 mM MgCl 2 5 mM coenzyme A (see recipe ) Radiolabeled carboxylic acid substrate (e.g., 100 µM 80

植物线粒体制备及其亚结构分级分离

.,  and  Braun,  H.  P.(2003) Fatty acid biosynthesis in mitochondria of grasses: malonyl-coenzyme A isgenerated  by  a  mitochondrial-localized  acetyl-coenzyme  A  carboxylase.  PlantPhysiol.  133,  875-884. 11.  Eubel, H., Jansch, L., and Braun