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文献和实验Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos Cheng Cui1 , Brenda Rongish1 , Charles Little1 , and Rusty Lansford2 ,3 1 Department of Anatomy and Cell Biology, University of Kansas Medical Center
) = 0.37g, H2 O = 1.0ml NaOH (1N) = 14µl; 2. solubilize in the boiling water bath (to heat ~1-3'; until pH will drop to ~7.0). PEG H(OCH2 CH2 )n OH; (store at 4o C). Conc. %(w/w) 10ml 50ml 100ml 150ml
) at the final concentration of 1 tablet/50ml): PBS Buffer A: 10 mM Hepes, pH 7.9, 10mM KCl, 1.5mM MgCl2, 0.5mM DTT S1 solution : 0.25 M Sucrose, 10 mM MgCl2 S2 solution: 0.35 M Sucrose, 0.5 mM MgCl2 S3 solution: 0.88 M Sucrose, 0.5 mM MgCl2
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