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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
Chromatin Immunoprecipitation (ChIP)
gel Antibodies to native protein of interest (or a post-translationally modified form) Antibody (control) Cell lysis buffer for ChIP (cold) Cells (50-100 million per experiment) Dilution buffer EDTA Elution
Microbiology - Serial Dilution
. +900 mL Buf. +900 mL Buf. +900 mL Buf. Yield: 1000 mL 10-1 1000 mL 10-2 1000 mL 10-3 1000 mL 10-4 4. Since 100 mL of the 10-1 dilution is used to make the 10-2 dilution, only 900 mL remains for use. 5. The buffer used for dilution
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