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文献和实验Immunohistochemistry (IHC) of Thymus Frozen Sections
µL of 2º antibody at r.t. as before x5 min. Rinse with PBS as before. Add DAB solution at r.t. x5 min. Rinse in PBS 2x. Step slides through graded EtOH to dehydrate: 70%, 95%, 100% (x3). Toluene (or xylene) (x2). Add
IHC frozen sections staining protocol
, methanol or ethanol) at -20°C for 30 min. (Abcam recommends starting with acetone) 3. Fix with the pre cooled fixative for 5-10 min, at room temperature. 4. Rinse 3-4 X in PBS. 5. Continue with the immunohistochemical staining protocol
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
(Boehringer Mannheim Cat# 604 205, 812 854)- Triton X-100 (Sigma Cat# X-100) - X-phosphate: 5-Bromo-4-chloro-3-indolyl-phosphate. (Boehringer Mannheim Cat# 1383 221) B. Stock solutions DEPC treatment of solutions is not needed. Solutions are stored
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