Corning特约代理商|3960 96-well Clea
r V-Bottom 2 mL Polypropylene Deep Well Plate, 5 per Bag, Sterile- ￥3000
- Greiner
- 780285
- 2025年07月14日
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上海玉博生物科技有限公司
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文献和实验slots from the underside in order to penetrate to the reservoir on the inflow and outflow ends. 4) At the end of each top plate, drill a centrally located 10/32" tracks per inch (T.P.I.) hole for polypropylene 1/8" hose barb to penetrate
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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