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Fastfilter Plasmid Maxi Kit Spin Protocol

the flow-through and reuse the collection tube in Step 11. 11. Add 10 ml Buffer HB to the Maxi column and centrifuge as above. This step ensures that residual protein contamination is removed and must be included for downstream applications requiring

Fastfilter Plasmid Midi Kit Spin Protocol

and centrifuge as above. This step ensures that residual protein contamination is removed and must be included for downstream applications requiring high quality DNA. Discard flow-through liquid and reuse the collection tube in the next step. 12. Wash

E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol

as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol