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- St John's Laboratory
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- 2025年07月08日
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文献和实验according to the instructions described in the Product Information Sheet provided with your microspheres.2.Transfer 5.0 x 106 of the stock microspheres to a USA Scientific microcentrifuge tube.3.Pellet the stock microspheres by microcentrifugation at ≥ 8000 x g for 1-2
Small scale DNA preps for Neurospora crassa.
to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex
Small scale DNA preps for Neurospora crassa.
, or until the cultures are saturated. 2. Spin the culture tubes for five minutes to compress the mycelia, then invert and blot the tubes on a paper towel to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S
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