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- 2025年07月12日
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文献和实验Microglia Isolation from Adult Mouse Brain
to isolate intact microglia from adult mouse brain for functional assays, immunocytochemistry, and/or flow cytometry analysis. This protocol involves enzymatic dissociation in medium supplemented with dispase II, papain, and DNase I followed by mechanical
General Protocol to Prepare Neuronal Cultures
every 3-4 days. Additional media and media supplements will need to be purchased to culture neurons past 4-6 days. Materials Needed Not Provide • Poly-D-lysine (Sigma P6407) for substrate • Papain (Sigma P4762; or Worthington) for enzymatic dissociation
are the Worthington product Codes: TL, TRL, TRLS, and TRLVMF. These products are suitable for cell harvesting as well as tissue dissociation. Elastase Pancreatic elastase is a serine protease with a specificity for peptide bonds adjacent
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