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上海玉博生物科技有限公司
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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
: 11. Can preincubate in Buffer A (1% BSA) at RT for a few hours. 12. Spin down worms gently and incubate with 1 in Buffer A (30 ul affinity purified anti-lin-14 antibody -preadsorbed with 292 vector protein- straight or diluted 1:1 or 1:2) for 24
Polyclonal Antibody Production
so nothing leaks out. Repeat for all four sites and place the rabbit back in its cage. Now do your other rabbits. Injections will be done every 4-6 weeks, with bleeds 7-10 days after each injection. Bleeds are performed identically
. Be aware that the chosen protein must not react with any of the affinity reagents to be used (e.g. rabbit serum cannot be used with protein A gold; bovine serum (FCS) cannot be used as a blocking agent if anti-BSA labelling is being studied).
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