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文献和实验A novel method of growing fungi for DNA extraction
of ''reverse agar'' (BASF pluronic polyol F-127), a block polymer of polyoxypropylene and polyoxyethylene, are solid at normal room temperatures, but liquid at 4C. When the compound is used as a replacement for agar in solid media, its unusual properties allow
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Purification (Method #1, or see Section F for Method #2) 1. Disassemble the gel running apparatus and transfer the gel to Saran Wrap. Apply radiolabeled or phosphorescent ink dots to the Saran Wrap for alignment. 2. Determine the labeled
Tissue sectioning: Cryostat sectioning method
here for a few days). * Remove sucrose/gelatin solution and add Tissue-Tek OCT (Miles Scientific, Naperville, IL 60566), compound, allow to equilibrate for at least 30 min (it can stay in there longer). * Prepare a block using Tissue-Tek.
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