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文献和实验A safe method of extracting DNA from Coccidioides immitis
nitrogen, lyophilized, and ground with a mortar and pestle. About 10 mg of dry ground tissue is mixed with 400 ul lysis buffer (50 mM Tris, 100 uM NaCl, 5 mM EDTA, 1 sodium dodecyl sulfate), and incubated at 80 C for 10 min., cooled to 40 C and incubated
Extraction of genomic DNA from whole blood
Author: Laura-Lee Boodram Source: Laura-Lee Boodram, Department of Life Sciences, The University of the West Indies Abstract: The protocol is simple and fairly rapid. It does not require the use of organic solvents but rather utilizes salt
Extraction of genomic DNA from whole blood
Remove DNA with a wide bore tip and transfer to a microfuge tube. Wash with 1 ml of 70% ethanol. Leave DNA to dry for 15-20 minutes at 37o C. Re-suspend in 300-400 µl of Tris HCl, pH 8.5 (not TE!). Leave to re-dissolve overnight at room
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