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文献和实验Immuno-Laser Capture Microdissection
with spot sizes of 50 to 60 µm (C) and 15 to 20 µm (D) in diameter, as seen during LCM without coverslip. The smaller spot size allows avoidance of interspersed T cells. Magnification x100 (C) and x400 (D). Fig. E: Lymph node stained
1. Human embryonic stem cells express a unique set of microRNAs • ARTICLE Developmental Biology, Volume 270, Issue 2, 15 June 2004, Pages 488-498 Mi-Ra Suh, Yoontae Lee, Jung Yeon Kim, Soo-Kyoung Kim, Sung-Hwan Moon, Ji Yeon Lee, Kwang-Yul Cha
Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae
) Day 1 Formaldehyde cross-linking Add 1.5 ml 36% formaldehyde to 50 ml of yeast cell culture (1% final) Shake slowly at 100 rpm at 30°C for 15 minutes Add 2.5 ml 2.5 M glycine Shake slowly at 100 rpm at 30°C
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