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文献和实验Competent cells, calcium chloride method, E. coli, description
, CaCl2 method, E. coli, long protocol Keywords: competent cells; E. coli; calcium chloride; Zappe CaCl2 competent cells Description Short protocol Related Contents Contributor: : Zappe, H
Sauer:Lysing E. coli with Lysozymes
. Hen Egg White Lysozyme I prepare the lysozyme fresh each time from lyophilized powder. Pre-warm the bottle to prevent moisture from condensing in the bottle when it''s opened. Pure B-Per and most lysis buffers don''t seem to allow
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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