sigma特约代理商|03708985001 Trypsin

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

of interestSodium citrate (50 mM, pH 6.0)TPCK-modified trypsin (sequence-grade)EquipmentCompact reaction columns (CRC) and filters (35-µm pore size)Incubator (slow rotation, e.g., rotating wheel)MALDI mass spectrometer (e.g., the Voyager DE-STR) or equivalentThe

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)

by tank with regulator; at least 1000 psi pressure) Iodoacetamide (IAA) Modified trypsin (sequencing grade) (optional, see Step 7) Poroszyme-immobilized trypsin (Applied Biosystems) (Optional, see Step 7) Soluble protein fractions If proteins

免疫沉淀-蛋白印迹法

& Medicine, King College London, UK).Rainbow recombinant protein molecular protein markers, ECL western blotting detection reagents were purchased from Amersham Pharmacia Boutech (Little Chalfont, Buckinghamshire, UK).Protogel 30%acrylamide solution, 10