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文献和实验Natural History Museum Protocol for EST sequencing from lamb
(1) Core out a single, well separated plaque from the agar plate using a 200µl pipette tip and place it into 500µl of SM buffer in a 1.5ml microtube: SM buffer 5.8g of NaCl 2.0g of MgSO4.7H2O 50ml of 1M Tris HCl (pH 7.5) 5ml of 2% Gelatin
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
, well separated plaque from the agar plate using a 200µl pipette tip and place it into 500µl of SM buffer in a 1.5ml microtube: SM buffer 5.8g of NaCl 2.0g of MgSO4.7H2O 50ml of 1M Tris HCl (pH 7.5) 5ml of 2% Gelatin solution
filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
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