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文献和实验Assay to Detect Lipid Peroxidation upon Exposure to Nanoparticles
hydroperoxides, and aldehydes, such as MDA, can be measured via a thiobarbituric acid reactive substances (TBARS) assay. In this assay, which can be performed in cell culture or in cell lysate, MDA combines with thiobarbituric acid (TBA) to form a fluorescent
mediated-condensation products of aldehydes with thiobarbituric acid in the thiobarbituric acid-reactive substances (TBARs) assay. Although this method will provide an overall measure of aldehyde levels, including malondialdehyde (MDA), it is plagued
Clonality - X Chromosome Inactivation Assay
. Clonal cell populations will show "loss" of the non-methylated allele after restriction digest. The assay can be performed on DNA recovered from microdissected samples. Both frozen tissue and fixed-embed allowfullscreen='true'ded archival tissue
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