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文献和实验Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
phase. On day of injection, spin 1-2 xE6 cells per mouse. Resuspend in IMDM or PBS at 1-2 xE6 cells/0.5ml (do not leave the cells without serum for more than 1 - 2 hr). Transfer cells to syringe using a 16G needle. Inject 0.5ml
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