Kingfisher Biotech特约代理商|DIY125

Sandwich ELISA Protocol

4 2.0 g KCl Add ddH2O up to 10 L, pH to 7.2 with HCl PBS/Tween: 0.5 ml of Tween-20 in 1 L PBS Blocking Solution: 10% fetal bovine serum or 1% BSA in PBS. Filter before use to remove particulates

ELISA操作指南

There are several other methods to wash an ELISA plate.Whatever you choose, be consistent with your washing technique throughout the whole process.        ELISA板还有其它几种洗涤方法。无论使用哪种方法，请在整个ELISA实验过程中保持一致 Phase 2: Blocking non-specific binding        第二阶段：封闭非特异性结合 Bovine

Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF

Bicarbonate 2.93 gSodium Carbonate 1.71 gDistilled H2O up to 1 literWASHING BUFFER10% Tween 20 ml10 mM PBS 4 litersBLOCKING BUFFER10 mM PBS 99 mlFCS 1 mlSAMPLE BUFFER0.5% BSA (Albumin Bovine) 2.5 g.0001% or 1 µg/ml Aprotinin .0005 g0.1 mM Benzethonium Chloride