Miltenyi特约代理商|130-041-407 Pre-

Screening BAC filters with non-radioactive probes

-hybridization and 5 ml for probe solution.)   Pre-hybridization of filters: more than 30 min at 42C After pre-hybridization, transfer the hybridization solution to 50 ml tube, add labeled probe (48 ml), mix

Dual- and Triple-Color Fluorospot

should preferably be filtered (0.2 μm). 2. Blocking: Lower membrane autofluorescence is obtained if blocking is performed with PBS containing 10% FCS instead of complete medium. 3. Serum in cell medium: A serum pre-evaluated for cell culture

类器官培养与应用——肾类器官

的分枝尖端，形成帽状间充质，促进帽状间充质中的肾祖细胞增殖分化，形成聚集(Pre-tubular aggregate，PA)。随后，PA 和 UB 分别进行间充质-上皮细胞转化（Mesenchymal-epithelial transition, MET）和小管生成，进而形成肾单位【2】。目前广泛用于肾脏研究的人肾脏细胞系忽略了细胞外微环境，与在体的器官类型和整体特性仍存在较大差异，不能充分体现出肾脏的作用及其特点，而三维培养的肾类器官模型为肾脏的研究提供了新途径。人源多能干细胞衍生的肾脏类器官