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文献和实验Revised protocol for establishing a mammary cell line from tumors
Revised protocol for establishing a mammary cell line from tumors Drench mouse in 70% ethanol, pin and dissect open Sterile dissection of tumor- leave some behind to use in histology Place tumor in small culture dish Weigh
问：BAF cell的来源是什么？有什么特性和用途呢？答：ATCC上没有你所谓的BAF cell，DSMZ上有两种BAF cell，如下：Cell line:BA-F8Cell type:mouse hybridoma(anti-rat fiber/myosin)Origin:this hybridoma cell line was described to secrete a mouse monoclonal antibody(IgG2b)that reacts with rat type
of antibodies, which might negatively effect cell growth.Our first procedure will involve the simple transfer of an established culture from a suspension culture. An aliquot will be removed from a commercially available cell line and transferred to prepared
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