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10
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上海玉博生物科技有限公司
GE特约代理商|17-0853-01 NAP-5色谱柱
NAP DNA columns are disposable DNA purification size exclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.
For rapid and efficient purification of DNA and oligonucleotides (≥ 10-mers) in less than 15 minutes by gravity flow
Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG/ICP Biocide
Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL (NAP-25)
Useful for small-scale purification, oligo purification, desalting, and buffer exchange
NAP DNA purification columns with Sephadex G-25 DNA Grade can be used for any DNA greater than 10 bases in length. These disposable DNA purification columns are ideal for the purification of oligonucleotides or very small DNA fragments following synthesis or a DNA labeling reaction, and for downstream applications such as PCR amplification and sequencing.
Each package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration, washing, and sample elution.
Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.
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文献和实验IL-25(又称IL-17E)是IL-17细胞因子家族成员,主要由活化的Th2细胞、肥大细胞、嗜碱性粒细胞及呼吸道、胃肠道和皮肤的上皮细胞分泌。其分子结构具有IL-17家族典型的半胱氨酸结。IL-25通过与由IL-17RA和IL-17RB组成的异源二聚体受体结合发挥功能,其中IL-17RB为其特异性识别亚基,主要表达于Th2细胞、嗜酸性粒细胞、肥大细胞及部分上皮和免疫细胞表面,在2型炎症反应中起关键调控作用。 01 IL-25 表达和结构 人类IL-25基因定位于14号染色体q11.2区域
.g. 20% ethanol, at neutral pH. 9. Ordering Information Product Pack size Code No. Benzamidine Sepharose 6B 25 ml 17-0568-01 10. References 1. Purification and characterization of a trypsin-like serine proteinase from rat brain slices
). Add DEPC treated water to make up to 9µl (4.5µl if using 10pmoles/µl primer). Add 1µl T4 polynucleotide kinase from same kit. Incubate for 30 minutes at 37°C. Remove top and bottom cap from NAP-5 column (Amersham-Pharmacia Biotech
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