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上海玉博生物科技有限公司
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文献和实验concentration of 20 to 200 micrograms/ml Prepare standards containing a range of 20 to 200 micrograms protein (albumin or gamma globulin are recommended) to a standard volume (generally 1 ml or less). See how to prepare and use a protein standard
or gamma globulin are recommended)in 100 µl volume.See how to set up an assay for suggestions as to setting up the standards.5.Add 5 ml dye reagent and incubate 5 min.6.Measure the absorbance at 595 nm.AnalysisPrepare a standard curve of absorbance
Reagent Bovine serum albumin (BSA) Spectrophotometer and tubes PROCEDURE Prepare standard dilutions of BSA containing 1, 2.5, 5.0, 7.5 and 10 mg/ml protein. Prepare serial dilutions of the unknown samples.
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