MBL特约代理商|5323 Ab-Match Assembl

Mapping, PCR DNA Sequencing

of Haemophilus influenzae, Methanococcus jannaschii, and Mycoplasma genitalium Celera Genomics demonstrated the ability to shotgun sequence the entire human genome (?)  Human Genome Assembly The HGP vs. Celera race to sequence the entire human genome

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

containing the human Lhcgr gene into a 170 kb BAC clone comprising the entire mouse orthologue. The methodology is based on the rationale to introduce a counter-selection cassette flanked by unique restriction sites and HAs for the insert, into the vector

在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官

。类器官包埋染色可用于基于抗体的鉴定。在该类器官染色方案中，我们详细介绍了通过免疫荧光共聚焦显微镜分析固定、渗透和染色包埋类器官的方法。 图1。使用抗体的类器官免疫荧光检测。使用乙酰-α-微管蛋白(A)、α-碳酸酐酶IV (B)和Sox-9 (AB5535) (C)抗体染色的人上皮肠类器官和肺类器官。 类器官染色方案 从类器官培养中移除培养基，用1X PBS (D8537)轻轻清洗2遍。 用15-20 mL 4% PFA (1.00496)在10厘米的培养皿中固定30-40个类器官ECM