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文献和实验实验原理 Dynabeads® CD3⁄CD28 CTS™ offer a simple method for isolation, activation and expansion of human T cells. Firstly, CD3 T cells are separated and concentrated from the apheresis product by magnetic cell separation
Activation and Expansion of Human Treg Cells
1: PBS w/ 0.1% BSA, pH 7.4 Cultural Medium: OpTmizer? T-Cell Expansion SFM (Gibco) serum free 1X formulation designed to support the culture and expansion of human T cells(or equivalent) Round bottom tissue culture plates or tissue culture flasks
Differentiating Neural Stem Cells into Neurons and Glial Cells
Medium Antibiotic-Antimycotic solution Fetal Bovine Serum, ES Cell-Qualified FBS GlutaMAX™-I FGF-basic (AA 10–155), Recombinant Human (bFGF) EGF, Recombinant Human CELLstart™ CTS™ Geltrex™ Reduced Growth Factor
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