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文献和实验Sodium Acetate Precipitation of Small Nucleic Acids
实验步骤 1. Add 2 μl carrier to the nucleic acid solution and mix well. 2. Add 1:10 volume of 3 M sodium acetate and mix thoroughly; for 230 μl of Lower Running Buffer, this will be 23 μl of 3 M
Assembly of Nucleosomal Templates by Salt Dialysis
) recipeHigh‐salt buffer (see recipe ) with 2 M NaCl in place of KCl recipeLow‐salt buffer (see recipe ) with 250 mM NaCl in place of KCl
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. 0.5% glutaraldehyde Make from a 25.0% stock immediately before use. You can buy a 25% solution (Sigma) and freeze-thaw it many times. 4.0% paraformaldyde 4 g paraformaldehyde 2 mM MgCl2 (0.2 ml of a 1 M stock) 1.25 mM EGTA (0.25 ml of a .5M
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