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文献和实验. no. 621722) Creatine kinase (Roche, cat. no. 127566) ATP (AppliChem, cat. no. A1348) HEPES (Gerbu Biotechnik, cat. no. 1009) Magnesium acetate (Merck, cat. no. 1.05819) 1,4-DTT (Roth, cat. no. 6908.2)
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
mM EGTA 25 µl of 1 M KCl 10 µl of 100 mM MgCl2 0.5 µl of 1 M DTT - 5 µl of 200 mM ATP in water 10 µl of 500 mM phosphocreatine in water 10 µl of 2.5 mg/ml creatine kinase in KPM buffer
Chromatin Assembly Using Drosophila Systems
) recipe0.5 M creatine phosphate (see recipe ) recipe5 mg/ml creatine kinase (see recipe ) 100 mM
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